Comparison of Helicobacter pylori Detection Using Immunohistochemistry and Giemsa and Its Association with Morphological Changes in Active Chronic Gastritis

Authors

  • Wildawati Nurdin Faculty of Medicine, University of Indonesia/Dr. Cipto Mangunkusumo Hospital, Jakarta
  • Ening Krisnuhoni Division of Gastroentero-hepatology, Department of Patology Anatomy, Faculty of Medicine, University of Indonesia/Dr. Cipto Mangunkusumo Hospital, Jakarta
  • Kusmardi Kusmardi Division of Gastroentero-hepatology, Department of Patology Anatomy, Faculty of Medicine, University of Indonesia/Dr. Cipto Mangunkusumo Hospital, Jakarta

DOI:

https://doi.org/10.24871/171201621-27

Keywords:

active chronic gastritis, H. pylori immunohistochemistry

Abstract

Background: Gastritis is an inflammation of the gastric mucosa as a response to infection or irritation of the gaster. The most common aetiology of chronic gastritis is Helicobacter pylori (H. pylori) infection. Presence of H. pylori is associated with the occurrence of inflammation, atrophy, and intestinal metaplasia. In terms of morphology, H. pylori is known in 2 forms, which are rod-shaped and coccoid-shaped. Coccoid-shaped bacteria are difficult to detect using Giemsa staining. Therefore, immunohistochemistry staining of H. pylori and evaluation of the sensitivity of coccoid-shaped of H. pylori are needed.Method: Cross-sectional study on 90 biopsy tissues of chronic gastritis patients in year 2015 and 2014, which included 30 Giemsa cases with positive H. pylori, 30 cases of active chronic gastritis with negative H. pylori but coccoid-shaped was found, and 30 non-active chronic gastritis, were subsequently stained with immunohistochemistry staining of H. pylori.Results: Expression of coccoid-shaped H. pylori in active chronic gastritis was significantly different (p < 0.05) in immunohistochemistry staining. There was a significant difference between active chronic gastritis with positive H. pylori and negative H. pylori in immunohistochemistry staining with degree of inflammation. Sensitivity and specificity test between Giemsa and immunohistochemistry staining showed sensitivity of 65% and specificity of 100%.Conclusion: Immunohistochemistry staining in active chronic gastritis was more sensitive compared to Giemsa staining in detecting H. pylori, particularly the coccoid-shaped bacteria.

Downloads

Published

2016-10-17

How to Cite

Nurdin, W., Krisnuhoni, E., & Kusmardi, K. (2016). Comparison of Helicobacter pylori Detection Using Immunohistochemistry and Giemsa and Its Association with Morphological Changes in Active Chronic Gastritis. The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy, 17(1), 21–27. https://doi.org/10.24871/171201621-27

Issue

Vol. 17 No. 1 (2016): VOLUME 17, NUMBER 1, April 2016

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).