Effect of Steamed Broccoli Juice (Brassica oleraceae L. var. italica) to the Serum Interleukin 8 Level in Colitis Murine Model

Authors

  • Lukas Mulyono Samuel Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Christian Maranatha/Immanuel Hospital, Bandung
  • Francisca AS Faculty of Medicine, University of Christian Maranatha, Bandung
  • Khie Khiong Department of Biology, Faculty of Medicine, University of Christian Maranatha, Bandung

DOI:

https://doi.org/10.24871/1632015148-152

Keywords:

ulcerative colitis, sulforaphane, interleukin-8

Abstract

Background: Ulcerative colitis (UC) is a bowel inflammation that happens in the colon and rectum. In UC there is an increased level of inflammatory mediators, one of which is interleukin-8 (IL-8), the main chemoattractant for polymorphonuclear (PMN) inflammatory cells causing inflammation to worsen. Broccoli (Brassica oleraceae L. var. italica) contains sulforaphane which can inhibit nuclear factor kappa B (NF-ĸB), resulting in the decrease of IL-8. This study was performed to identify the effect of steamed broccoli juice to the serum interleukin-8 level in murine model of colitis.

Method: This study was a true experimental laboratory study using complete randomized design. Experimental animals being used were 28 murines with Balb/c strain and were divided into 4 intervention groups. Serum IL-8 level was measured by using enzyme-linked immunosorbent assay (ELISA) method. Data analysis was performed using analysis of variance (ANOVA) test, followed by multiple comparisons Tukey honest significant difference (HSD) test (a = 0.05) and p  0.05.

Results: The highest mean of IL-8 level was found in the positive control group. This study also showed highly significant difference (p < 0.001) of IL-8 level between positive control group and treatment group.

Conclusion: Steamed broccoli juice could decrease serum interleukin-8 level in murine model of colitis.

Downloads

Published

2015-12-01

How to Cite

Samuel, L. M., AS, F., & Khiong, K. (2015). Effect of Steamed Broccoli Juice (Brassica oleraceae L. var. italica) to the Serum Interleukin 8 Level in Colitis Murine Model. The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy, 16(3), 148–152. https://doi.org/10.24871/1632015148-152

Issue

Vol. 16 No. 3 (2015): VOLUME 16, NUMBER 3, December 2015

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).