Detection of the Helicobacter pylori caga gene in gastric biopsies from dyspeptic patients using PCR method in Mataram General Hospital

Authors

  • Soewignyo Soemodihardjo

DOI:

https://doi.org/10.24871/1120008-9

Abstract

A polymerase chain reaction assay (PCR) for the diagnosis of Helicobacter pylori in human gastric biopsies from 44 dyspeptic patients was developed. From endoscopic diagnosis, the sample consisted of 4 patients with gastric cancer, 4 patients with duodenal ulcer, 3 patients with ventricular ulcer, 3 patients with both ventricular ulcer and duodenal ulcer and 31 patients with chronic gastritis. The H. pylori infection was diagnosed using nested PCR, which consisted of two primers of the urease gene fragment. From 44 specimens with urease positive, 39 specimens were positive for cagA strain (88,6%). This reflects a very high frequency of the cagA gene in the Helicobacter pylori infection. The meaning of this is still unclear. There was no significant difference in the cagA status of ulcer dyspeptic patient and non- ulcer dyspeptic patients. 
Key words: CagA gene, Helicobacter pylori, PCR
VOLUME 1, ISSUE 1, December 2000

Downloads

Published

2000-01-12

How to Cite

Soemodihardjo, S. (2000). Detection of the Helicobacter pylori caga gene in gastric biopsies from dyspeptic patients using PCR method in Mataram General Hospital. The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy, 1(1), 8–9. https://doi.org/10.24871/1120008-9

Issue

VOLUME 1, NUMBER 1, December 2000

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).